Human minisatellite MS32 (D1S8) displays somatic but not germline instability in transgenic mice
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Human minisatellite MS32 (D1S8) displays somatic but not germline instability in transgenic mice.
Human minisatellite MS32 (D1S8) shows instability both in the germline and, at much lower levels, in somatic DNA. To investigate factors that influence somatic and germline mutation, large cosmid-based constructs containing MS32 were introduced into mice, bred to homozygosity and tested for instability in blood and sperm. Analysis of single copy and multicopy transgenic lines revealed somatic m...
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The human hypervariable minisatellite MS32 has a well characterised internal repeat unit array and high mutation rates have been observed at this locus. Analysis of MS32 mutants has shown that male germline mutations are polarised to one end of the array and frequently involve complex gene conversion-like events, suggesting that tandem repeat instability may be modulated by cis-acting sequences...
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Mutation at most human minisatellites is driven by complex interallelic processes that give rise to a high degree of length polymorphism and internal structural variation. MSY1, the only highly variable minisatellite on the non-recombining region of the Y chromosome, is constitutively haploid and therefore precluded from interallelic interactions, yet maintains high diversity in both length and...
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1. Minisatellites, originally developed for forensic DNA fingerprinting and profiling, have also provided extremely informative systems for analysing processes of tandem repeat DNA turnover in the human genome. 2. Minisatellite instability involves distinct mutation processes in somatic and germline cells. In sperm, tandem repeat instability most likely arises at meiosis as a by-product of high...
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ژورنال
عنوان ژورنال: Human Molecular Genetics
سال: 1997
ISSN: 1460-2083
DOI: 10.1093/hmg/6.9.1565